nih3t3 (ATCC)
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99
Structured Review
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nih3t3
Nih3t3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2387 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nih3t3+mouse+embryonic+fibroblasts/NIH%2F3T3%3B+Embryonic+Fibroblast%3B+Mouse/pmc12799909-18-0-2
Average 99 stars, based on 2387 article reviews
Nih3t3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2387 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nih3t3+mouse+embryonic+fibroblasts/NIH%2F3T3%3B+Embryonic+Fibroblast%3B+Mouse/pmc12799909-18-0-2
Average 99 stars, based on 2387 article reviews
nih3t3 - by Bioz Stars,
2026-11
99/100 stars
Images
1) Product Images from "Protocol to identify mechanosensitive nuclear proteins using tunable actomyosin contractility and proximity biotinylation in mammalian cells"
Article Title: Protocol to identify mechanosensitive nuclear proteins using tunable actomyosin contractility and proximity biotinylation in mammalian cells
Journal: STAR Protocols
doi: 10.1016/j.xpro.2025.104288
Figure Legend Snippet: Mechanosensitive responses of PTBP1 under various mechanical conditions (A) Volcano plot demonstrating significant nuclear enrichment of PTBP1 following tetracycline treatment (FDR< 0.01). Reprinted data with permission from Tseng et al. (B) Western blot analysis of PTBP1 nuclear enrichment, with YAP as a positive control. (C) Diagram shows the mechanical conditions used in this protocol to verify PTBP1 expression. Immunofluorescence microscopy confirming the nuclear accumulation of PTBP1 induced by high mechanical tension including low cell density (D, scale bar: 20 μm), stiff substrate (E, scale bar: 10 μm), and mechanical stretching (F, scale bar: 50 μm). (G) Immunofluorescence images showing PTBP1 nuclear reduction in ML-7 treated NIH3T3. Bar represents 10 μm.
Techniques Used: Western Blot, Positive Control, Expressing, Immunofluorescence, Microscopy
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